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Image Search Results
Journal: Oncogenesis
Article Title: Activated platelet-derived exosomal LRG1 promotes multiple myeloma cell growth
doi: 10.1038/s41389-024-00522-5
Figure Lengend Snippet: A Proteomic analysis of platelet-rich plasma derived from healthy individuals or MM patients, and a heat map showing the differential proteins. B GO analysis was performed on the differential proteins, and cell component analysis found that the differential proteins were closely related to vesicle secretion. C GSEA analysis of differential proteins found that differential proteins were closely related to EMT and angiogenesis. D Volcano plot showing significantly different proteins found in proteome analysis. E The expression of S100A8, S100A9, MPO, SAA1, and LRG1 in platelet-rich plasma derived from healthy people or patients with MM was verified by WB, and the expression of LRG1 was significantly increased in platelet-rich plasma derived from patients with MM. F The exosomes in platelet-rich plasma derived from MM patients were obtained, and the morphology and particle size of exosomes were analyzed by transmission electron microscopy and nanoparticle tracking analysis (NTA). G Exosomes expressing the specific platelet marker CD41 and exosome marker CD63 were sorted by flow cytometry. H The expression of LRG1 in platelet-derived exosomes was verified by WB. Compared with the control group, LRG1 expression was significantly increased in MM platelet-derived exosomes. *** P < 0.001.
Article Snippet:
Techniques: Clinical Proteomics, Derivative Assay, Expressing, Transmission Assay, Electron Microscopy, Marker, Flow Cytometry, Control